Skills & Expertise
Detail Directed Problem Solver — Ability to direct complex biological / healthcare projects from concept to a fully operational state.
Enzyme Assay Design & Development, Upstream & Downstream Processing, Chromatography, TFF, HCS, Flow Cytometry, etc.
Excellent Communication Skills: Conveying scientific information with power and precision, Outstanding Presentation Skills.
Creating Engaging Presentations, Active Listening, Customer Centricity, Pre- & Post - Installation Training & Demonstrations.
Skilled in Statistics and Data Analysis, DOE, Meta-Analysis, SPSS, MS Excel, Power Bi, GraphPad Prism, Design-Expert, etc.
Competitive Intelligence, CRM data Management, Competition Analysis, Product positioning for Research equipment, etc.
Fermentation Technology: Optimization & Screening of Fermentation Medium, Optimization of Process parameters for Enzymes (protease and Staphylokinase). Alcohol Production on Bench Scale Fermenter. Handling of fermenters from New Brunswick, Applikon, Scigenics and CHEMAP from (5-500 litres).
Downstream Processing: Preparative and analytical column chromatography, ion-exchange, hydrophobic interaction, Expanded-bed chromatography, from 2ml - 10L columns. Bead-milling at bench scale, (Batch and Continuous mode), Ultrasonication, Microfiltration and Ultra filtration (Lab-scale to Large scale) etc.
Microbiology: Isolation, Characterization, Maintenance and preservation of bacterial cultures, Screening of Microorganisms to produce Enzymes, etc.
Enzyme Technology & Immuno-diagnostics: Optimization of Enzyme Activity & Reaction Conditions, Determination of V- max, Km value & Molecular weight of enzymes. DRID, SRID, Rocket Immuno-Electrophoresis, Counter Immuno-Electrophoresis, ASLO and WIDAL tests.
Protein Crystallization: Proficient in handling High-throughput and automated Protein Crystallization systems, Designing of screen and screening of proteins for crystallization.
Biosensors and High Content Screening: Acoustic based biosensors, laser based scanning system.
Automated Robotics: Ability to handle automated liquid handling systems and Nano-liter robots.
Flow Cytometry: Multiplexing and Ploidy- analysis. Multi-colour experiments, CD3, CD4, CD8, CD45 Counting, etc.
Instrumentation techniques: Electrophoresis (Agarose gel, SDS & Native PAGE), Protein crystallization, Screen preparation, Band quantification Spectrophotometry, luminometry, HPLC, Gas Chromatography, Batch & continuous centrifugation, French press, High-Pressure cell homogenizers.
Computers: Working and troubleshooting hardware, software. Can work with Design-Expert, SPSS, Graphpad Prism, MacOSX, iWork, iOS, Windows Vista, XP, Me & 98, with knowledge of networking, LAN, WLAN and all major office and scientific / academic software.